ESR 12: Fat-free diet for mitochondria: Mushroom-enriched diets for NAFLD

Project Summary

This project aims at using a mouse model for Non-Alcoholic Fatty Liver Disease (NAFLD)/ Nonalcoholic steatohepatitis (NASH) to demonstrate that a diet enriched in mushrooms alleviates the disease phenotype through improvement of mitochondrial metabolism, when compared with a regular diet.

Research will include a wide variety of techniques ranging from basic cell biology, isolation of pure and intact mitochondrial fractions from several tissues, measurement of mitochondrial function through multiple techniques, mtDNA copy number, including using the Seahorse XFe96 platform, Western Blotting and qRT-PCR, SPECCs technology for mid-range IR profiling in mouse blood samples and isolation of mitochondrial-associated membranes.

Host institution

German Research Center for Environmental Health (HMGU), Germany


Dr. Hans Zischka


Center for Neuroscience and Cell Biology (CNC), Portugal

Nencki Institute of Experimental Biology (NENCKI), Poland


Dr. Paulo Oliveira

Dr. Anabela Marisa Azul

Dr. Mariuz Wieckowski